Proteinuria and hypertension are independent factors affecting fetal DNA values: a retrospective analysis of affected and unaffected patients.

controlled multivariate or longitudinal study settings would be imperative. The agreement between the two methods was evaluated by correlating results from patient samples and control solutions of the two assays, and a Bland-Altman plot was constructed to visualize the differences (Fig. 1). Despite the fairly good correlation, the trend observed in the Bland–Altman plot together with the large S y͉x (2.19 mg/L for the samples) prevent direct comparison of the results. The Bland–Altman plot (Fig. 1) further indicates that the difference is composed of both constant and proportional components. To further evaluate the nature of the differences, we performed a Deming regression analysis because there are neither commutable primary calibrators nor reference methods available to evaluate the trueness of results of adiponectin measurements. In the Deming regression analysis, both patient and control samples were included, and it yielded a slope of 0.7670 (SE ϭ 0.0352), an intercept of Ϫ2.740 (0.8557) mg/L, and a correlation coefficient (r) of 0.9420 (P Ͻ0.0001). The overall imprecision in duplicate measures as demonstrated by the constant SD was 0.627 mg/L for the ELISA and 1.761 mg/L for the RIA methods (Deming analysis). These results indicate similar dynamic performances, but clear differences in calibration between the methods. The surprisingly large variation between duplicate measurements on the RIA method possibly caused the Deming regression slope to be slightly better (0.7670 vs 0.7161) and the correlation coefficient slightly weaker (r ϭ 0.9420 vs 0.9673) than those of the traditional correlation analysis (patients and controls together). Together with the practical disadvantages associated with the use of radiolabeled reagents, this may be interpreted as an advantage for the ELISA method over the RIA-based method in the research laboratory. In conclusion, this study confirmed that the enzyme immunometric assay evaluated here is a robust and an easily implementable tool for measuring adiponectin concentrations on a standard platform in clinical laboratory research. The Fujirebio Inc. (Tokyo, Japan) made this study possible by kindly donating the reagents needed to perform the adiponectin ELISA assay. cDNA cloning and expression of a novel adipose specific collagen-like factor, apM1 (adipose most abundant gene transcript 1). Hyperadiponectinemia in obesity and type 2 diabetes: close associatin with insulin resistance and hyperinsulinemia. et al. Association of adiponectin mutation with type 2 diabetes. Circulating adiponectin levels are reduced in non-obese but insulin-resistant first-degree relatives of type 2 diabetic patients. Plasma adiponectin concentrations predict insulin sensitivity of both glucose and lipid …